Purification, Crystallization and Properties of Tryptophanase fromProteus rettgeri

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Properties of Crystalline Tryptophanase.

In 1903 Hopkins and Cole (1) identified tryptophan as the source of indole produced by bacterial cultures. The tryptophan-inducible enzyme responsible for this conversion, which is found most commonly in Escherichia coli, was subsequently named tryptophanase by Happold and Hoyle (2). Wood, Gunsalus, and Umbreit (3) showed that tryptophanase catalyzed the stoichiometric conversion of tryptophan ...

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The tryptophanase-tryptophan reaction. 9. The nature, characteristics and partial purification of the tryptophanase complex.

The name tryptophanase was given by Happold & Hoyle (1935) to the enzyme complex of Escherichia coli which induces and catalyzes the production of indole from tryptophan by non-viable bacterial preparations with the consumption of five atoms of oxygen (Woods, 1935). The present communication describes the preparation of this complex in the cell-free state, and subsequent investigation of the co...

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ژورنال

عنوان ژورنال: Agricultural and Biological Chemistry

سال: 1974

ISSN: 0002-1369

DOI: 10.1080/00021369.1974.10861469